dot hybridization meaning in Chinese

斑点杂交

It indicated that 76 . 5 percent of positive frequency of all the plants was achieved by using southern dot hybridization , 87 . 5 percent of the plants with gus positive activity was gotten by pcr southern blotting and 42 percent of the plants with gus positive activity was gotten by southern blotting
转基因卡那霉素抗性植株中， gus表达阳性率为43 ，随机抽样的方法进行杀虫肽和npt基因的pcr检测阳性率均为100 。经过分子生物学检测表明， southern斑点杂交76 . 5植株表现阳性。
The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna , which was first linearized by aocl . after two rounds of plaque isolation , the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization . the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10
重组转移载体dna与经bsu36i酶切线性化的修饰型病毒bm - bacpakdna共转染家蚕bmn细胞，两轮空斑筛选和纯化，获得重组病毒rbmbacph - egf ，经pcr扩增、 dna点杂交等方法鉴定证实重组病毒中已正确插入ph - egf融合基因。